metN [2019-09-02 13:38:21]

methionine [SW|ABC transporter ](ATP-binding protein)

locus: BSU_32750

pI: 7.30

mw: 37.73 kDa

protein length: 341 aa

gene length: 1026 bp

function: methionine uptake

product: methionine [SW|ABC transporter ](ATP-binding protein)

essential: no

ec: 3.6.3.-

synonyms: yusC

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.2|Uptake of amino acids]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,363,266 → 3,364,291

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

Structure

• [PDB|3DHW] (the [protein|D7254B7F31D3F4CD18172AFB0F526CAE5195BDB6|MetN]-[protein|F94070DD039A422055FFC35087FB87765F22E156|MetP] complex from ''E. coli'', 44% identity) [Pubmed|18621668]

[SW|Localization]

• associated to the cell membrane, via [protein|F94070DD039A422055FFC35087FB87765F22E156|MetP] [Pubmed|10092453,18763711]

Expression and Regulation

Operons

• genes: [gene|D7254B7F31D3F4CD18172AFB0F526CAE5195BDB6|metN]-[gene|F94070DD039A422055FFC35087FB87765F22E156|metP]-[gene|0481A9C134A6EEC6F111AD0C03E2E345D6A315A0|metQ]
  description: [Pubmed|10094622]

  regulatory mechanism

  • [regulon|S-box|S-box]: transcription termination/ antitermination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination, in [regulon|S-box|S-box]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: activation, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • activated during growth in the presence of branched chain amino acids ([protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]) [Pubmed|12618455]
  • the mRNA is processed between [gene|F94070DD039A422055FFC35087FB87765F22E156|metP] and [gene|0481A9C134A6EEC6F111AD0C03E2E345D6A315A0|metQ] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
  • the [SW|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• MGNA-A596 (yusC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/596 NBRP B. subtilis, Japan]
• 1A797 ( ''metN''::''cat''), [Pubmed|14990259], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A797&Search=1A797 BGSC]
• BKE32750 (Δ[gene|D7254B7F31D3F4CD18172AFB0F526CAE5195BDB6|metN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAGCAATCACCTGCCTTA, downstream forward: _UP4_CAGGTGAAAGCAGAGGTGAT
• BKK32750 (Δ[gene|D7254B7F31D3F4CD18172AFB0F526CAE5195BDB6|metN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAGCAATCACCTGCCTTA, downstream forward: _UP4_CAGGTGAAAGCAGAGGTGAT

References

• 10092453,14990259,12618455,18763711,10094622,18039762,18621668,29794222